Presentation Fluorimeter

Presentation

Fluorescence spectroscopy is a powerful technique that allows analysis at equilibrium or in real time, changes in the environment of intrinsic or extrinsic fluorescent probes (including tryptophan for protein, synthetic fluorophores).
The Biophysical Platform has a high sensitivity fluorometer multimodular (Quantamaster QM4CW ; Manufacturer : Photon Technology International) equipped with several modules to measure the fluorescence steady state in normal or polarized light (fluorescence anisotropy measurements) or the lifetime of fluorescence.
Two types of quantities can be quantified with the instrument: the intensity and polarization (or anisotropy) of fluorescence, static measurements (equilibrium) or dynamic ("stopped-flow).

Applications:

The technology can be applied both to the study of:

• conformational stability of macromolecules
• characterization of molecular interactions

Staff

Manager: Caroline Mas
Office: CIBB room 020
Platform: CIBB room 001

Specific Equipment

Quantamaster QM4CW from Photon Technology International

Access mode

Users must read the conditions of use of the instruments as well as of the management of the services provided:

• The platform is accessible to local PSB researchers, external academics (Instruct) and industrials

Cost

Academics: Participation to the maintenance fees
Industrials: Please contact us for cost information

Location

The Biophysical platform is located in the Carl-Ivar Brändén Building, ground floor, room 001 (Shared Building C).

How to make a request

To make a booking request, fill this Request form

Fluorimeter booking

Samples

Only non-pathogenic biological samples are accepted. Decree of the July 18, 1994 establishing the list of biological pathogens, amended by Decrees of April 17, 1997 and June 30, 1998 (Decrees in French). The list of biological pathogens is available on the website of the IPBS.

Follow up / acknowledgements

The user agrees to promote the obtained results by mentioning the platform in the acknowledgements in case of publication or scientific communication and to communicate the reference of the article to the responsible for the platform.

Service / Poviding access mode: Acknowledgements in publications and oral presentations

Acknowledgements in publications:

Please find below the sentence that has to be written in all publications based on our UAR platforms:
"This work used the platforms of the Grenoble Instruct-ERIC center (ISBG ; UAR 3518 CNRS-CEA-UGA-EMBL) within the Grenoble Partnership for Structural Biology (PSB), supported by FRISBI (ANR-10-INBS-0005-02) and GRAL, financed within the University Grenoble Alpes graduate school (Ecoles Universitaires de Recherche) CBH-EUR-GS (ANR-17-EURE-0003). We thank Caroline Mas for assistance and/or access to the biophysics platform."