Presentation OMNISEC

OMNISEC couples an exclusion chromatography and different detectors, static light scattering, viscometer, refractive index and absorbance. OMNISEC combines the separation of macromolecules and their characterization. It allows :

• Molecular weight, concentration and hydrodynamic radius determination
• Quantifying the homogeneity of the preparations
• Determining the stoichiometry of complexes, including for complex systems associating protein-detergent, protein-RNA, protein-polymer. It is a method often appropriated for studying interactions of membrane protein and/or glycosylated proteins.
The platform is committed to the establishment of a quality approach implementation that resulted in June 2011 with the ISO 9001 (2008) and in July 2015 with NF X50-900 (2015).

OMNISEC

Application

• Determination of concentration, molecular weight and hydrodynamic radius
• Determination of aggregation and oligomerisation states, homogeneity of the sample
• Determination of conformational changes : interactions
• It is a method often appropriated for studying interactions of membrane protein and/or glycosylated proteins

Key words

Size-exclusion chromatography, Laser Light Scattering, Protein concentration, Oligomerisation states, stoichiometry of complexes

Staff

Manager : Caroline Mas
Operated by : Caroline Mas and Aline Le Roy
Office : CIBB room 017 / IBS room 355
Platform : CIBB room 001

Specific Equipment

OMNISEC (MALVERN)

• Detectors : RALS, LALS, UV (PDA), RI, Viscosimeter
• Auto sampler ; temperature regulation 4-40°
• Column oven : 25-35 °C
• Detector oven : 25-35 °C
• Column : Superdex 200, 75 and 30 10/300, Superose 6 10/300 GL (GE Healthcare)

Used software : OMNISEC
The software is freely available here.

Access mode

Open to academics, which will be trained to data analysis.
Open to academics and private companies for the realisation of the analysis, on a service or collaboration mode, depending on equipment and staff availablility.

How to make a request ?

Each new project manager should contact should contact : Caroline Mas and Aline Le Roy . Each user will get trained to the analysis and agrees to comply with the general terms and commitment.
All applications should be accompanied by a request form. The applicant should fill the first part, which is completed in agreement by both part, in order to define the best way to conduct the experiments, before signature of the parts. A local contact will be associated and available for the users for the duration of the use of OMNISEC, for advices and assistance. If samples are sent, or stored by the plateform, storing is as defined by the applicant in the request form.

OMNISEC Request Form

Booking Calendar

Cost

Academics : Participation to the maintenance fees
Industrials : Please contact us for cost information

Follow up / acknowledgements

The user agrees to promote the obtained results by mentioning the platform in the acknowledgements in case of publication or scientific communication and to communicate the reference of the article to the responsible for the platform.

Service / Providing access mode : Acknowledgements in publications and oral presentations

Collaboration : Co-authors in publications and oral presentations.

Acknowledgements in publications :

Please find below the sentence that has to be written in all publications based on our UAR platforms :
"This work used the platforms of the Grenoble Instruct-ERIC center (ISBG ; UAR 3518 CNRS-CEA-UGA-EMBL) within the Grenoble Partnership for Structural Biology (PSB), supported by FRISBI (ANR-10-INBS-0005-02) and GRAL, financed within the University Grenoble Alpes graduate school (Ecoles Universitaires de Recherche) CBH-EUR-GS (ANR-17-EURE-0003). We thank Caroline Mas for assistance and/or access to the biophysics platform."