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Integrated Structural Biology Grenoble
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High Throughput Membrane Protein Crystallisation

Presentation

HTMPC platform is fully equipped to accomplish successful crystallization of your membrane protein (in meso, bicelle, vapor diffusion). Robotic systems nanovolume dispenser (NT8 from Formulatrix) allows to screen 96 precipitant conditions with small amount of sample (5-10 µl per plate) in less than 10 minutes. Our platform works with commercially available kits specialized in membrane protein crystallization. An automated imaging system, RockImager 1500 (Formulatrix), visualizes crystallization drops with visible, polarized and UV lights, which allows a better identification of protein crystals. The smallest crystals which can be confidently detected are about 10 micrometers.

The diffusion rate and mobile fraction of the membrane protein can be determined by FRAP (Formulatrix), that allows to identify the optimal conditions for LCP crystal growth. In addition, FTIR (Brucker) and UV-Vis specrophotometer (Schimadzu) are available at the platform for precise determination of detergent and membrane protein content correspondingly.

Key words

Structural Biology, high-throughput crystallization, in meso crystallization, membrane proteins, lipidic cubic phase ; LCP-FRAP, FT-IR

Contact

ibs-plateforme-htmpc.contact ibs.fr

Dedicated staff

  • Dupeux Florine

Specific equipment

Robot for in meso membrane protein crystallization (nanodispenser), automated system for imaging crystallization plates, UV microscope for visualisation of protein crystals, temperature controlled rooms for crystallisation (22°C, 12°C, 4°C), CRIMS to visualize images through internet.

Access mode

Open access to internal and external users.
https://www-crims-htmpc.ibs.fr (login : crimsUser ; pwd : 6horowitz)

Cost

⚠️ <html>https://www-crims-htmpc.ibs.fr/index.php?option=com_content&view=article&id=49&Itemid=395</html>

Location

IBS/Room 313
IBS/Office 357

How to make a request ?

Register and send request forms on our website : https://www-crims-htmpc.ibs.fr
(login : crimsUser ; pwd : 6horowitz)

Credits

In the general case users must aknowledge the platform in their publications : “This work used the platforms of the Grenoble Instruct-ERIC center (ISBG ; UMS 3518 CNRS-CEA-UGA-EMBL) within the Grenoble Partnership for Structural Biology (PSB), supported by FRISBI (ANR-10-INBS-05-02) and GRAL, financed within the University Grenoble Alpes graduate school (Ecoles Universitaires de Recherche) CBH-EUR-GS (ANR-17-EURE-0003)."