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Integrated Structural Biology Grenoble

Contact person(s) related to this article / Caroline Mas

Microscale Thermophoresis

Definition

Microscale Thermophoresis is a method to quantify biomolecular interactions. It measures the motion of molecules along microscopic temperature gradients and detects changes in their hydration shell, charge or size. By combining the fluorescence detection with thermophoresis, MST provides a way to measure molecular interactions.

Principle

When performing a MST experiment, a microscopic temperature gradient is induced by an IR laser, and the movement of molecules is detected and quantified using either covalently attached dyes, fluorescent fusion proteins or intrinsic tryptophan fluorescence.

MST Principle

Applications

The applications range from small-molecule binding events to protein-protein interactions and interactions of multi-protein complexes.

Staff

Platform engineer: Caroline Mas
Office: CIBB room 020
Instrument location: EMBL room 152

Instrument

Nanotemper Monolith NT.115 Green/Red

Manual NT.115
MST Starting guide

Location

The MST Monolith NT.115 is located at the EMBL, laboratory 152, 1st Floor (Map Here)

Access mode

Instrument allocation after user training:

  • It is mandatory to attend a training session before using this instrument.
  • The first two days of use will be done with the assistance of the manager.
  • After demonstrating his autonomy, the user will be able to use the instrument in complete independence
  • When needed, please ask to the platform manager
  • The platform is accessible to local PSB researchers, external academics (Instruct) and industrials
  • The platform manager will provide assistance for:
    • _ experimental design
    • _ samples consideration
    • _ data collection
    • _ data analysis
  • Contact the platform manage to arrange a meeting
Fiche Prestation


The platform is now providing consumables, please contact Caroline Mas for more information.

Capillaries Standard (200 Count)
Premium Capillaries (200 count)
Aaliquot Labeling Kit RED-NHS 2nd generation
Aliquot Labeling Kit RED-MALEIMIDE 2nd generation
Kit His-Tag Labeling Kit RED-tris-NTA 2nd generation

Cost

Academics: Participation to themaintenance fees
Industrials: Please contact us for cost information

How to make a request

To make a booking request, fill this request form here
You can also access to the booking system on the PSB website

MST Monolith NT.115 Booking

Follow up / acknowledgements

Acknowledgements in publications:
Please find below the sentence that has to be written in all publications based on our UMS platforms:
"This work used the platforms of the Grenoble Instruct-ERIC center (ISBG ; UMS 3518 CNRS-CEA-UGA-EMBL) within the Grenoble Partnership for Structural Biology (PSB), supported by FRISBI (ANR-10-INBS-05-02) and GRAL, financed within the University Grenoble Alpes graduate school (Ecoles Universitaires de Recherche) CBH-EUR-GS (ANR-17-EURE-0003). We thank Caroline Mas for assistance and/or access to the Biophysical platform."