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Integrated Structural Biology Grenoble

Contact person(s) related to this article / Caroline Mas

Microscale Thermophoresis


Microscale Thermophoresis is a method to quantify biomolecular interactions. It measures the motion of molecules along microscopic temperature gradients and detects changes in their hydration shell, charge or size. By combining the fluorescence detection with thermophoresis, MST provides a way to measure molecular interactions.


When performing a MST experiment, a microscopic temperature gradient is induced by an IR laser, and the movement of molecules is detected and quantified using either covalently attached dyes, fluorescent fusion proteins or intrinsic tryptophan fluorescence.

MST Principle


The applications range from small-molecule binding events to protein-protein interactions and interactions of multi-protein complexes.


Platform engineer: Caroline Mas
Office: CIBB room 020
Instrument location: EMBL room 152


Nanotemper Monolith NT.115 Green/Red

Manual NT.115
MST Starting guide


The MST Monolith NT.115 is located at the EMBL, laboratory 152, 1st Floor (Map Here)

Access mode

Fiche Prestation
  • The platform is accessible to local PSB researchers, external academics (Instruct) and industrials
  • The platform manager will provide assistance for:
    • _ experimental design
    • _ samples consideration
    • _ data collection
    • _ data analysis
  • Contact the platform manage to arrange a meeting
  • Training on the instrument is provided the day of your first experiment

The platform is now providing consumables, please contact Caroline Mas for more information.

Capillaries Standard (200 Count)
Premium Capillaries (200 count)
Aaliquot Labeling Kit RED-NHS 2nd generation
Aliquot Labeling Kit RED-MALEIMIDE 2nd generation
Kit His-Tag Labeling Kit RED-tris-NTA 2nd generation


Academics: Participation to themaintenance fees
Industrials: Please contact us for cost information

How to make a request

To make a booking request, fill this request form here
You can also access to the booking system on the PSB website

MST Monolith NT.115 Booking

Follow up / acknowledgements

Acknowledgements in publications:
Please find below the sentence that has to be written in all publications based on our UMS platforms:
"This work used the platforms of the Grenoble Instruct-ERIC center (ISBG ; UMS 3518 CNRS-CEA-UGA-EMBL) within the Grenoble Partnership for Structural Biology (PSB), supported by FRISBI (ANR-10-INBS-05-02) and GRAL, financed within the University Grenoble Alpes graduate school (Ecoles Universitaires de Recherche) CBH-EUR-GS (ANR-17-EURE-0003). We thank Caroline Mas for assistance and/or access to the Biophysical platform."