Home > Biophysics characterisation > Microscale Thermophoresis (MST) > Microscale Thermophoresis (MST)
Microscale ThermophoresisDefinitionMicroscale Thermophoresis is a method to quantify biomolecular interactions. It measures the motion of molecules along microscopic temperature gradients and detects changes in their hydration shell, charge or size. By combining the fluorescence detection with thermophoresis, MST provides a way to measure molecular interactions. PrincipleWhen performing a MST experiment, a microscopic temperature gradient is induced by an IR laser, and the movement of molecules is detected and quantified using either covalently attached dyes, fluorescent fusion proteins or intrinsic tryptophan fluorescence.
ApplicationsThe applications range from small-molecule binding events to protein-protein interactions and interactions of multi-protein complexes. StaffManager: Caroline Mas InstrumentNanotemper Monolith NT.115 Green/Red
LocationThe MST Monolith NT.115 is located at the EMBL, laboratory 152, 1st Floor (Map Here) Access modeUsers must read the conditions of use of the instruments as well as of the management of the services provided: Instrument allocation after user training:
The platform can provides consumables for new projects.
CostAcademics: Participation to themaintenance fees and MST consumables are not included and are at the charge of the users. How to make a requestTo make a booking request, fill this request form here MST Monolith NT.115 BookingAcknowledgements in publications: |