Cellular imaging by electron microscopy
Introduction
Different techniques are available in the platform to allow the observation of biological samples
1. Fixation
- Chemical fixation
- Fixation by high-pressure freezing
2. Inclusion
- Classical embedding (EPON, LR White)
- Cryo-substitution and embedding
3. Sectioning
- Classical sectioning
- Cryo-sectioning (Tokuyasu)
4. Labeling and Staining
- Post-staining
- Immunolabeling (manual or automated)
5. Observation
- Trasmission Electron Microscopy
Keywords
Cellular Transmission Electron Microscopy, High-Pressure Freezing, cryo-substitution, ultra-thin sections, immuno-labeling.
Dedicated Staff
• Benoit Gallet
• Daphna Fenel
• Christine Moriscot
• Guy Schoehn
• High-pressure freezer Leica HPM100
• Freeze-substitution machine Leica AFS2 + FSP
• (Cryo-)ultra-microtome Leica UC7/FC7
• Immuno-labeling machine Leica IGL
Accessibility and cost
Access for PSB members, other academia and industry (please refer to costs).
How to contact us?
ibs-plateforme-em.contact ibs.fr
Sample types
Cell monolayer or pellet, bacteria, or tissue.
Samples have to be at the biohazard level L1.
L2 samples must be chemically fixed before.
Results
The results allow:
- Morphological studies of cells and bacteria
- Protein localization at a sub-cellular level
- Determination of virus infection cycle
Development of correlative microscopy is in progress.
Data Storage
Data are stored for 3 years.
Credits
If you use the results obtained (with the platform or in collaboration), please add:
In your acknowledgements:
« This work used the platforms of the Grenoble Instruct-ERIC center (ISBG ; UAR 3518 CNRS-CEA-UGA-EMBL) within the Grenoble Partnership for Structural Biology (PSB), supported by FRISBI (ANR-10-INBS-0005-02) and GRAL, financed within the University Grenoble Alpes graduate school (Ecoles Universitaires de Recherche) CBH-EUR-GS (ANR-17-EURE-0003). The electron microscope facility is supported by the Rhône-Alpes Region, the Fondation Recherche Medicale (FRM), the fonds FEDER, the Centre National de la Recherche Scientifique (CNRS), the CEA, the University of Grenoble, EMBL, and the GIS-Infrastrutures en Biologie Sante et Agronomie (IBISA). »
In your publications:
Gallet Benoit1,2,3, Fenel Daphna1,2,3, Moriscot Christine1,2,3, Schoehn Guy1,2,3
1CEA, Institut de Biologie Structurale Jean-Pierre Ebel, UMR5075 Grenoble France.
2CNRS, Institut de Biologie Structurale Jean-Pierre Ebel, UMR5075 Grenoble France.
3Université Grenoble Alpes, Institut de Biologie Structurale Jean-Pierre Ebel, UMR5075 Grenoble France.
Location
IBS, ground and 1st floor, Methods and Electron Microscopy Group.
Quality
The platform is certified ISO9001-NFX 50-900.
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