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Integrated Structural Biology Grenoble
Accueil > Cellular electron microscopy

Contacts relatifs à cet article / Benoit Gallet / NEUMANN Emmanuelle

Cellular imaging by electron microscopy


Different techniques are available in the platform to allow the observation of biological samples

1. Fixation

  • Chemical fixation
  • Fixation by high-pressure freezing

2. Inclusion

  • Classical embedding (EPON, LR White)
  • Cryo-substitution and embedding

3. Sectioning

  • Classical sectioning
  • Cryo-sectioning (Tokuyasu)

4. Labeling and Staining

  • Post-staining
  • Immunolabeling (manual or automated)

5. Observation

  • Trasmission Electron Microscopy


Cellular Transmission Electron Microscopy, High-Pressure Freezing, cryo-substitution, ultra-thin sections, immuno-labeling.

Dedicated Staff

• Benoit Gallet
• Daphna Fenel
• Christine Moriscot
Guy Schoehn


• High-pressure freezer Leica HPM100
• Freeze-substitution machine Leica AFS2 + FSP
• (Cryo-)ultra-microtome Leica UC7/FC7
• Immuno-labeling machine Leica IGL

Accessibility and cost

Access for PSB members, other academia and industry (please refer to costs).

How to contact us ?

Sample types

Cell monolayer or pellet, bacteria, or tissue.
Samples have to be at the biohazard level L1.
L2 samples must be chemically fixed before.


The results allow :

  • Morphological studies of cells and bacteria
  • Protein localization at a sub-cellular level
  • Determination of virus infection cycle

Development of correlative microscopy is in progress.

Data Storage

Data are stored for 3 years.


If you use the results obtained (with the platform or in collaboration), please add :
In your acknowledgements :
« This work used the platforms of the Grenoble Instruct-ERIC center (ISBG ; UAR 3518 CNRS-CEA-UGA-EMBL) within the Grenoble Partnership for Structural Biology (PSB), supported by FRISBI (ANR-10-INBS-0005-02) and GRAL, financed within the University Grenoble Alpes graduate school (Ecoles Universitaires de Recherche) CBH-EUR-GS (ANR-17-EURE-0003). The electron microscope facility is supported by the Rhône-Alpes Region, the Fondation Recherche Medicale (FRM), the fonds FEDER, the Centre National de la Recherche Scientifique (CNRS), the CEA, the University of Grenoble, EMBL, and the GIS-Infrastrutures en Biologie Sante et Agronomie (IBISA). »
In your publications :
Gallet Benoit1,2,3, Fenel Daphna1,2,3, Moriscot Christine1,2,3, Schoehn Guy1,2,3
1CEA, Institut de Biologie Structurale Jean-Pierre Ebel, UMR5075 Grenoble France.
2CNRS, Institut de Biologie Structurale Jean-Pierre Ebel, UMR5075 Grenoble France.
3Université Grenoble Alpes, Institut de Biologie Structurale Jean-Pierre Ebel, UMR5075 Grenoble France.


IBS, ground and 1st floor, Methods and Electron Microscopy Group.


The platform is certified ISO9001-NFX 50-900.