Cryo-electron microscopy

Summary

The cryo-electron microscopes, and the associated instruments, are available under conditions.

The EM platform also offers other services.

Key word

Cryo-electron microscopy

Dedicated staff

• Emmanuelle Neumann
• Guy Schoehn

Specific equipment

• Cryo-electron microscope FEI Tecnai F20 200 kV (FEG).
• Cryo-electron microscope Thermo Scientific GLACIOS 200 kV FEG.

Accessibility

The platform is accessible to members of the PSB, academia, and industry.

How to contact us?

ibs-plateforme-em.contact ibs.fr

Additional information

The protein has to be bigger than 300 kDa and pure (SDS-PAGE gel). Try to avoid detergent and glycerol. 30 microliters at 1 mg/ml + buffer should be enough.

Costs

See prices

Credit

If you use these results (from the service platform or collaboration) please add:

• In your acknowledgments:
  « This work used the EM facilities at the Grenoble Instruct-ERIC Center (ISBG; UAR 3518 CNRS CEA-UGA-EMBL) with support from the French Infrastructure for Integrated Structural Biology (FRISBI; ANR-10-INSB-0005-02) and GRAL, a project of the University Grenoble Alpes graduate school (Ecoles Universitaires de Recherche) CBH-EUR-GS (ANR-17-EURE-0003) within the Grenoble Partnership for Structural Biology. The IBS Electron Microscope facility is supported by the Auvergne Rhône-Alpes Region, the Fonds Feder, the Fondation pour la Recherche Médicale and GIS-IBiSA.»
• In your publications:
  Neumann Emmanuelle1,2,3; Schoehn, Guy1,2,3
  1CEA, Institut de Biologie Structurale Jean-Pierre Ebel, UMR5075 Grenoble France.
  2CNRS, Institut de Biologie Structurale Jean-Pierre Ebel, UMR5075 Grenoble France.
  3Université Grenoble Alpes, Institut de Biologie Structurale Jean-Pierre Ebel, UMR5075 Grenoble France.

Location

IBS, ground floor, electron microscopy and methods group.