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Integrated Structural Biology Grenoble
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Contact person(s) related to this article / Benoit Gallet / NEUMANN Emmanuelle

Cellular imaging by electron microscopy

Introduction

Different techniques are available in the platform to allow the observation of biological samples

1. Fixation

  • Chemical fixation
  • Fixation by high-pressure freezing

2. Inclusion

  • Classical embedding (EPON, LR White)
  • Cryo-substitution and embedding

3. Sectioning

  • Classical sectioning
  • Cryo-sectioning (Tokuyasu)

4. Labeling and Staining

  • Post-staining
  • Immunolabeling (manual or automated)

5. Observation

  • Trasmission Electron Microscopy

Keywords

Cellular Transmission Electron Microscopy, High-Pressure Freezing, cryo-substitution, ultra-thin sections, immuno-labeling.

Dedicated Staff

• Benoit Gallet
• Daphna Fenel
• Christine Moriscot
Guy Schoehn

Equipments

• High-pressure freezer Leica HPM100
• Freeze-substitution machine Leica AFS2 + FSP
• (Cryo-)ultra-microtome Leica UC7/FC7
• Immuno-labeling machine Leica IGL

Accessibility and cost

Access for PSB members, other academia and industry (please refer to costs).

How to contact us?

ibs-plateforme-em.contact ibs.fr

Sample types

Cell monolayer or pellet, bacteria, or tissue.
Samples have to be at the biohazard level L1.
L2 samples must be chemically fixed before.

Results

The results allow:

  • Morphological studies of cells and bacteria
  • Protein localization at a sub-cellular level
  • Determination of virus infection cycle

Development of correlative microscopy is in progress.

Data Storage

Data are stored for 3 years.

Credits

If you use the results obtained (with the platform or in collaboration), please add:
In your acknowledgements:
« This work used the platforms of the the Grenoble Instruct-ERIC Center (ISBG : UMS 3518 CNRS-CEA-UGA-EMBL) with support from FRISBI (ANR-10 INBS-05-02) and GRAL (ANR-10-LABX-49-01) within the Grenoble Partnership for Structural Biology (PSB). The electron microscope facility is supported by the Rhône-Alpes Region, the Fondation Recherche Medicale (FRM), the fonds FEDER, the Centre National de la Recherche Scientifique (CNRS), the CEA, the University of Grenoble, EMBL, and the GIS-Infrastrutures en Biologie Sante et Agronomie (IBISA). »
In your publications:
Gallet Benoit1,2,3, Fenel Daphna1,2,3, Moriscot Christine1,2,3, Schoehn Guy1,2,3
1CEA, Institut de Biologie Structurale Jean-Pierre Ebel, UMR5075 Grenoble France.
2CNRS, Institut de Biologie Structurale Jean-Pierre Ebel, UMR5075 Grenoble France.
3Université Grenoble Alpes, Institut de Biologie Structurale Jean-Pierre Ebel, UMR5075 Grenoble France.

Location

IBS, ground and 1st floor, Methods and Electron Microscopy Group.

Quality

The platform is certified ISO9001-NFX 50-900.