Presentation
UV-visible and Raman spectroscopy on nano-volume biological samples
Techniques:
– Absorption, Fluorescence, Fluorescence lifetime, Raman microspectrophotometry,
– Actinic (laser) excitation of photosensitive samples.
Samples:
– protein crystals or solutions from cryo- to room-temperatures.
Typical applications:
– Study of oxidation state in metalloproteins,
– Study of X-ray induced radiation damage,
– Study of coloured proteins or ligands in crystalline and solution states,
– Kinetic crystallography,
– Test bench for online spectroscopic experiments on the ESRF MX beamlines.
Key words
Microspectrophotometry, crystals, Raman, absorption, fluorescence, cryo.
Contact
Antoine Royant
Dedicated staff
– Antoine Royant
– Martin Weik (on-line microspec)
Specific equipment
Lasers at 266 nm, 355 nm, 405 nm, 440 nm, 457 nm, 465 nm, 472 nm, 477 nm, 488 nm, 496 nm, 502 nm, 514 nm, 561 nm, 633 nm; Spectrometers ; Cryostream.
Website
www.esrf.eu/UsersAndScience/Experiments/MX/Cryobench/
Access mode
Open access. Support is provided to academic groups on a service basis for routine experiments and through collaboration for particular requirements or for repeated access.
Cost
Free of charge to academic users
Location
ESRF – ID29
How to make a request ?
e-mail proposal to antoine.royant@esrf.fr