Introduction
Different techniques are available in the platform to allow the observation of biological samples
1. Fixation
- Chemical fixation
- Fixation by high-pressure freezing
2. Inclusion
- Classical embedding (EPON, LR White)
- Cryo-substitution and embedding
3. Sectioning
- Classical sectioning
- Cryo-sectioning (Tokuyasu)
4. Labeling and Staining
- Post-staining
- Immunolabeling (manual or automated)
5. Observation
- Trasmission Electron Microscopy
Keywords
Cellular Transmission Electron Microscopy, High-Pressure Freezing, cryo-substitution, ultra-thin sections, immuno-labeling.
Dedicated Staff
• Benoit Gallet
• Daphna Fenel
• Christine Moriscot
• Guy Schoehn
Equipments
• High-pressure freezer Leica HPM100
• Freeze-substitution machine Leica AFS2 + FSP
• (Cryo-)ultra-microtome Leica UC7/FC7
• Immuno-labeling machine Leica IGL
Accessibility and cost
Access for PSB members, other academia and industry (please refer to costs).
How to contact us?
ibs-plateforme-em.contact@ibs.fr
Sample types
Cell monolayer or pellet, bacteria, or tissue.
Samples have to be at the biohazard level L1.
L2 samples must be chemically fixed before.
Results
The results allow:
- Morphological studies of cells and bacteria
- Protein localization at a sub-cellular level
- Determination of virus infection cycle
Development of correlative microscopy is in progress.
Data Storage
Data are stored for 3 years.
Credits
If you use the results obtained (with the platform or in collaboration), please add:
In your acknowledgements:
« This work used the platforms of the Grenoble Instruct-ERIC center (ISBG ; UAR 3518 CNRS-CEA-UGA-EMBL) within the Grenoble Partnership for Structural Biology (PSB), supported by FRISBI (ANR-10-INBS-0005-02) and GRAL, financed within the University Grenoble Alpes graduate school (Ecoles Universitaires de Recherche) CBH-EUR-GS (ANR-17-EURE-0003). The electron microscope facility is supported by the Rhône-Alpes Region, the Fondation Recherche Medicale (FRM), the fonds FEDER, the Centre National de la Recherche Scientifique (CNRS), the CEA, the University of Grenoble, EMBL, and the GIS-Infrastrutures en Biologie Sante et Agronomie (IBISA). »
In your publications:
Gallet Benoit1,2,3, Fenel Daphna1,2,3, Moriscot Christine1,2,3, Schoehn Guy1,2,3
1CEA, Institut de Biologie Structurale Jean-Pierre Ebel, UMR5075 Grenoble France.
2CNRS, Institut de Biologie Structurale Jean-Pierre Ebel, UMR5075 Grenoble France.
3Université Grenoble Alpes, Institut de Biologie Structurale Jean-Pierre Ebel, UMR5075 Grenoble France.
Location
IBS, ground and 1st floor, Methods and Electron Microscopy Group.
Quality
The platform is certified ISO9001-NFX 50-900.