CM02 team
Contact: cm02.contact@ibs.fr
Guy Schoehn
Scientific director
Gregory Effantin
Beamline Operations manager
Pauline Juyoux
Beamline scientist
Felix Weis
Beamline scientist
Eleftherios Zarkadas
Beamline scientist
Presentation
CM02 is a Titan Krios G4 (Thermo Fisher Scientific), equipped with a cold FEG, a Selectris X energy filter and a Falcon 4i electron detector. It is operated as an ESRF CRG beamline: one third of the beam time is allocated to ESRF users while the rest is available for French laboratories through a curated process. This cryo-electron microscope is able to carry out single-particle and tomography experiments. CM02 is part of the « France Cryo-EM » infrastructure financed by the “Plan Investissement d’Avenir 3” scheme which has enabled the purchase of 3 state-of-the-art 300 kV cryo-electron microscopes in France. It is maintained and operated by a team of 5 people from the IBS/ISBG (Grenoble).
Technical specification
- Microscope: ThermoFisher Scientific Titan Krios G4
- Accelerating voltage = 300 kV
- Electron source: Cold Field Emission Gun
- Fringe free illumination
- Detector: Falcon 4i camera
- Energy filter: Selectris X
- Phase plate: FEI Volta Phase Plate
- Cs = 2.7 mm
- Pixel size
| Magnification | Calibrated pixel size (Å) |
|---|---|
| 270 kx | 0,46 |
| 215 kx | 0,57 |
| 165 kx | 0,73 |
| 130 kx | 0,94 |
| 105 kx | 1,2 |
- Movies format: eer or tiff
- Acquisition software: EPU or TOMO 5
- Both remote and on-site sessions are possible.
Routes of access
We encourage all users to contact the CM02 team before submitting a proposal.
National CRG access (up to two third of total beam time)
Proposal template can be downloaded here and send to cm02.contact@ibs.fr
Cost
Microscope time is free of charge for 2025. All other expenses are the user’s responsibilities.
ESRF access (one third of total beam time)
Applications to use CM01 and CM02 Krios microscopes are made through the ESRF User Portal (SMIS). The type of proposal is a structural biology rolling-access application, which can be submitted at any time during the year. Additionally, specific cryo-EM BAGs have been created (which are distinct from the MX-BAGs). Contact: cryo-em@esrf.fr
Cost
For accepted proposals, travel expenses will be reimbursed for a maximum of two visitors per experiment.
Instruct-ERIC access
International access, including sample shipment and travel expenses, can be funded through Instruct-ERIC, see https://instruct-eric.org/centre/instruct-centre—france-2/
Industrial access
Contact us for a quote.
Sample requirement
Evidence of sample quality and suitability must be given to justify the use of this Titan Krios microscope.
- For SPA experiments, include results such as typical micrograph, 2D class averages, 3D reconstruction, atlas, etc.
- For molecular cryo-ET: Atlas and high magnification image of the sample.
- For cellular cryo-ET: Atlas and medium magnification images showing cells/lamellae with the regions of interest.
Grids must have been screened and clipped in autogrids compatible with the autoloader system. Up to 4 grids will be loaded on the microscope.
Experiments
Single particle analysis
Typical SPA sessions are 48h. On demand, 24h sessions can be allocated. In specific cases, sessions up to 72h can be offered if justified.
Several grids can be collected in one session with the multigrid acquisition strategy, with the same data collection parameters.
Tilted acquisition (up to 40°) can be considered if previous high resolution data acquisition analysis displayed strong preferential orientations not solved by sample preparation optimization.
Tomography
Tomography sessions can be of 48-72h depending on the needs.
Sending samples
For national CRG access and Instruct access
Please send your dry shipper to the following address:
IBS
[Local contact name] – MEM group
71 avenue des Martyrs
CS 10090
38044 Grenoble Cedex 9
France
Inform your local contact of the delivery and provide them with a tracking number. Include a return form to send the dry shipper back after the session.
For ESRF allocated access
https://www.esrf.fr/UsersAndScience/UserGuide/Preparing/ImportingEquipment
Retrieving data
Data can be downloaded directly from the ESRF servers.
See https://www.esrf.fr/Infrastructure/Computing/ComputingOffsite/FtpWindows
Acknowledgment
For national CRG experiments (CRGiiiii experiments) and in house experiments (IHiiiii experiments)
The cryo-electron microscopy data were acquired at the CM02 CRG beamline operated by IBS at the European Synchrotron Radiation Facility in Grenoble. The purchase of this microscope was funded by the EquipEx+ France Cryo-EM project (ANR-21-ESRE-0046). We thank [local contact] for performing the experiments and providing local support.
For ESRF beamtime (MXiiii experiments)
We acknowledge the European Synchrotron Radiation Facility for provision of beam time on CM02 CRG beamline. The purchase of this microscope was funded by the EquipEx+ France Cryo-EM project (ANR-21-ESRE-0046). We thank [local contact] for performing the experiments and providing local support.
Notes:
- There is currently no publication available to use as a reference.
- We encourage adding this extra sentence in the acknowledgments: “We thank Guy Schoehn for the establishment of the cryoEM facility.”
- We would also be grateful if you could acknowledge us in your other communications, such as talks and posters.
