Mass spectrometry
PRESENTATION
Keywords :
Protein analysis, peptide and small molecule analysis, High-Resolution Mass Spectrometry, Native Mass Spectrometry, LC-ESI-TOF, MALDI-TOF/TOF, limited proteolysis.
Services :
The mass spectrometry platform at IBS offers the following types of analysis :
- Determination of the mass of intact proteins, peptides and small molecules ;
- Control of protein and peptide expression, modification, mutation and labeling ;
- Analysis of protein limited proteolysis ;
- Sequencing of intact proteins by MALDI-Top Down Sequencing (MALDI-TDS) ;
- Analysis of protein complexes by native mass spectrometry.
The activities proposed by the mass spectrometry platform can be carried in two ways :
- Routine analyses (Quality Control) : corresponding to priced analyses, which do not require technical development ;
- Collaborative projects (Research and Development) : corresponding to analyses that require the development of a specific method and/or a technological development.
Accessibility :
The services offered are accessible to IBS research groups, PSB partners, and external academic and industrial applicants.
Quality approach :
The mass spectrometry platform has been engaged in developing quality management that led in June 2011 to the ISO 9001 (version 2008) certification. The quality management allows an optimal follow up of competences and equipment, of scientific information (traceability of data, analyses and projects), better visibility and transparency of used procedures and a continuous adequacy with the needs expressed by the users.
Cost :
Analyses prices are defined and adapted depending on the type of service and project requested by the customer. Contact us for a quote.
Location and contact :
IBS Building, ground-floor
Office : rooms 166 / 167
Laboratory : rooms 159 / 159A
Email : ibs-plateforme-ms.contact ibs.fr
ANALYSIS REQUEST AND ANALYSIS REPORT
How to make a request ?
1. Before submitting the analysis request form and if you have any questions concerning sample preparation and the choice of the more appropriate analysis method, we suggest you contacting us for discussion.
2. Fill out the analysis request form (AR) and send it by e-mail to ibs-plateforme-ms.contact ibs.fr.
 - Analysis request form (.doc)
3. A unique analysis number is assigned to each analysis request for sample identification. The samples are collected at IBS, room 166/167 (from Monday to Friday, between 9:30am and 5:30pm).
4. The analysis will start only after validation of the analysis request form by both the platform staff and the user (signing of the AR at sample reception).
Information regarding the samples :
Toxic and/or radioactive samples are not accepted. Only the biological samples whose biohazard level is 1 (non-pathogenic) will be accepted.
Optimal conditions for MS analysis :
- Sample concentration and volume : ≥ 10 microM and minimum volume 20 µl ;
- Use water of high quality for all solutions (HPLC or MilliQ quality) ;
- Buffers and additives : avoid phosphate (PBS) and sulphate buffers, detergents (e.g. Triton, CHAPS...) and contamination by polymers (e.g. polyethylene glycols) ;
- For samples into solution is recommended filtering or centrifuging prior to analysis in order to eliminate any insoluble substance and suspended particles ;
- Sample tubes must be clearly annotated with an ID corresponding to that reported on the AR form (refer to sample list).
Samples will be stored at room temperature, 4 ° C or -20 ° C (depending on the choice indicated on the AR form). The samples will not be stored after analysis unless specified by the client.
Analysis Report
The results (analysis report in pdf format) are available on the site PLAGE (http://plage.ibs.fr/plage2/) by logging in with your IBS session identifiers and using the tutorial :
 - Tutorial PLAGE (.pdf)
A precise deadline for the communication of the results is defined with the user on a case-by-case basis upon receipt of the samples. Analysis reports and raw data will be stored and available for 5 years.
DEDICATED STAFF AND TECHNICAL RESSOURCES
Staff :
Platform Manager : Elisabetta Boeri Erba, CEA research engineer
Luca Signor, CEA research engineer
Agneta Kiss, CNRS engineer assistant
Equipment :
- MALDI TOF/TOF MS (Autoflex maX, Bruker Daltonics)
ion source : MALDI
analyzer : TOF (linear and reflectron mode)
m/z up to 500’000 (linear mode)
resolution : up to > 26’000 (reflectron mode) and > 2’500 (linear mode) - LC ESI-TOF MS (6210, Agilent Technologies)
ion source : ESI et nanoESI (HPLC-Chip)
analyzer : TOF
m/z 30-3000
resolution : > 20’000
coupled to capillary and nano HPLC system (1100 series). - High mass ESI Q-TOF MS (Ultima, Micromass Waters)
ion source : ESI et nanoESI
analyzer : TOF
m/z 30-32000
resolution : > 10’000
coupled to capillary and nano HPLC system (CapLC Waters).
Instrument monitoring : the spectrometers are checked up regularly (preventive and corrective maintenance service contract) and are calibrated before each series of measurements.
The methods proposed in the context of quality control are detailed in the document attached herewith. Development and application of other methods of analysis by mass spectrometry will be possible after prior discussion with the staff of the platform.
- Analytical methods (.pdf)
FOLLOW-UP AND OUTCOMES- Routine analyses : the user agrees to promote the obtained results by mentioning the platform in the acknowledgements in case of publication or scientific communication. In the general case, users must acknowledge the platform in their publications by using the sentence :
« This work used the platforms of the Grenoble Instruct-ERIC center (ISBG ; UAR 3518 CNRS-CEA-UGA-EMBL) within the Grenoble Partnership for Structural Biology (PSB), supported by FRISBI (ANR-10-INBS-0005-02) and GRAL, financed within the University Grenoble Alpes graduate school (Ecoles Universitaires de Recherche) CBH-EUR-GS (ANR-17-EURE-0003) ». - Collaborative projects : collaborative projects imply that the platform staff that have been actively involved in the project will be co-authors of the published papers or communications.
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