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Integrated Structural Biology Grenoble

Contact person(s) related to this article / FERRER Jean-Luc

The FIP Beamline

Ligne FIP

Presentation

FIP-BM30A is the French ESRF beamline for protein crystallography. FIP-BM30A can be used either for single or multi-wavelength diffraction (SAD, MAD) experiments. Its optics deliver a focused beam on a fixed sample position, with an energy resolution of about 10-3 to 10-4 and a large accessible energy range (7-18 keV). The beam intensity is 0.5e11 photons/(0.3x0.3 mm2) for a 2.10-4 energy resolution at 12.5 keV.

FIP-BM30A is a highly automated beamline, with a unique robotized experiment setup. Indeed, apart the robotized sample changer, FIP posses also a plate screening system (G-Rob) for direct in situ analysis of crystals in the X-ray beam. With this system, it is possible to analyze crystals in sitting drop crystallization plates or microchips. This is specifically interesting for crystals too small to be fished, or too fragile to be handled or frozen. A rapid screening to determine the nature of the object to be analyzed (salt or protein crystals), collecting few frames for cell determination, or even complete data collection are possible.

Key words

protein crystallography, anomalous phasing

Contact

Jean-Luc Ferrer

Dedicated staff

  • 7 permanent staff(J. Joly, M. Pirocchi, C. Brezin, M.Terrien, F. Borel, P. Israel-Gouy, J.-L. Ferrer),
  • 5 local-contacts : 2 scientists from the “Synchrotron group” (D. Cobessi, E.Oksanen), and 3 scientists coming from other IBS groups (R. Kahn, E. Girard, C.Cavazza)

Specific equipment

FIP-BM30A is a bending-magnet beamline with:

  • symmetrical fixed exit optics: 2 mirrors and a double monochromator with cryogenic cooling and sagittal focusing), and soon an over-focusing optical device.
  • an experimental setup comprising: a MD2 goniometer with on-axis camera and mini-kappa, an ADSC 315r CCD detector, a Rontec MCA counter, a sample changer robot with the possibility to screen crystals directly in their crystallization plate.

Website

http://www.esrf.eu/UsersAndScience/Experiments/CRG/BM30A/

Access mode

FIP-BM30A is accessible to:

  • French academic users, through a national program committee,
  • European academic users, through the ESRF scientific review committee,
  • industrial users (direct access, no selection process, limited to a 10% quota).

Cost

The access to FIP-BM30A is free for academic users, and fee-based (rate fixed by the ESRF) for industrial users.

Location

FIP-BM30A is located at the ESRF, on bending magnet #30.

How to make a request ?

For French users and industrials, follow the common procedure FIP/Soleil :(http://sunset.synchrotron-soleil.fr/sun/)
For European users, follow the standard ESRF application procedure