Tecnai F20

Credit: A Delos CEA.

Specifications

  • Accelerating voltage: 200 kV
  • Electron source: C-FEG
  • CETA camera
  • ASI hybrid detector, 1K × 1K
  • SerialEM acquisition software
  • Gatan 626 cryogenic specimen holder
  • Gatan 614 cryo-tomography holder

Main Applications

The Tecnai F20 is a versatile cryo-equipped transmission electron microscope used for both room-temperature and cryogenic imaging.

Applications include:

  • Negative staining
  • Cellular electron microscopy
  • Cryogenic grid screening
  • Single-particle cryo-EM
  • Cryo-electron tomography
  • Observation of CEMOVIS sections
  • Tomography of cellular and molecular specimens
  • Microcrystal electron diffraction, or MicroED

The side-entry cryogenic holders allow vitrified specimens to be observed and tilt series to be acquired for tomographic reconstruction.