Specifications
- Accelerating voltage: 200 kV
- Electron source: X-FEG
- CETA camera
- Gatan K2 Summit direct electron detector
- Falcon 4i direct electron detector
- Automated cryogenic specimen loading
Available acquisition and imaging software includes:
- EPU 3 with multigrid acquisition
- Tomo 5
- MAPS
- Latitude S
- SerialEM
Main Applications
The Glacios is the platform’s principal instrument for cryogenic grid screening, sample optimization, and 200 kV cryo-EM data collection.
It is used for:
- Screening grids before high-resolution data collection on a Titan Krios
- Assessment of ice thickness and grid quality
- Evaluation of particle concentration and distribution
- Identification of aggregation or preferential particle orientation
- Automated single-particle data collection
- Molecular and cellular cryo-electron tomography
- Collection of preliminary datasets to demonstrate sample suitability
- High-resolution single-particle structure determination
Depending on the specimen and grid quality, the microscope can support near-atomic-resolution single-particle reconstructions. The platform has achieved reconstructions at resolutions down to approximately 2.2 Å.
The Glacios is also an important intermediate step between initial vitrification and advanced data collection on a 300 kV Titan Krios. Thermo Fisher Scientific similarly positions the Glacios as a 200 kV cryo-TEM for high-performance single-particle analysis, tomography, and pre-screening within integrated Glacios–Krios workflows.