Summary
The cryo-electron microscopes, and the associated instruments, are available under conditions.
The EM platform also offers other services.
Key word
Cryo-electron microscopy
Dedicated staff
– Emmanuelle Neumann
– Guy Schoehn
Specific equipment
– Cryo-electron microscope FEI Tecnai F20 200 kV (FEG).
– Cryo-electron microscope Thermo Scientific GLACIOS 200 kV FEG.
Accessibility
The platform is accessible to members of the PSB, academia, and industry.
How to contact us?
ibs-plateforme-em.contact@ibs.fr
Additional information
The protein has to be bigger than 300 kDa and pure (SDS-PAGE gel). Try to avoid detergent and glycerol. 30 microliters at 1 mg/ml + buffer should be enough.
Costs
See prices
Credit
If you use these results (from the service platform or collaboration) please add:
– In your acknowledgments:
« This work used the EM facilities at the Grenoble Instruct-ERIC Center (ISBG; UAR 3518 CNRS CEA-UGA-EMBL) with support from the French Infrastructure for Integrated Structural Biology (FRISBI; ANR-10-INSB-0005-02) and GRAL, a project of the University Grenoble Alpes graduate school (Ecoles Universitaires de Recherche) CBH-EUR-GS (ANR-17-EURE-0003) within the Grenoble Partnership for Structural Biology. The IBS Electron Microscope facility is supported by the Auvergne Rhône-Alpes Region, the Fonds Feder, the Fondation pour la Recherche Médicale and GIS-IBiSA.»
– In your publications:
Neumann Emmanuelle1,2,3; Schoehn, Guy1,2,3
1-CEA, Institut de Biologie Structurale Jean-Pierre Ebel, UMR5075 Grenoble France.
2-CNRS, Institut de Biologie Structurale Jean-Pierre Ebel, UMR5075 Grenoble France.
3-Université Grenoble Alpes, Institut de Biologie Structurale Jean-Pierre Ebel, UMR5075 Grenoble France.
Location
IBS, ground floor, electron microscopy and methods group.