PRESENTATION
The SPR/BLI platform offers a OctetRED96e instrument from Sartorius (former PALL/FortéBio) for characterizing biomolecular interactions using BioLayer Interferometry (BLI).
BLI is an optical technique that measures variations in optical thickness at the surface of a biosensor through interference created by the reflection of a light beam on the surface of the biosensor and on the surface of the biomolecular layer. This real-time, label-free method enables the detection of biological molecules.
With the OctetRED96e, this technology facilitates the study of interactions between partners and the determination of kinetic constants of association and dissociation (kon, koff), affinity constants (KD), and the quantification of biological molecules (concentration).
The method involves a biosensor on which a ligand is immobilized; the binding of an analyte is measured on the biosensor’s surface. The response is recorded in real-time (sensorgram).
This technique is applicable to characterizing molecular interactions involving small molecules and various classes of biological macromolecules, including proteins, polysaccharides, lipids, and nucleic acids.
ACCESS, LOCATION, BOOKING, RATES, AND CONTACTS
Access
The BLI platform is available to:
- Academic Users: Access modalities (service access, autonomous use, or collaboration) are defined based on the involvement of platform staff.
- Industrial Users: Services are provided on a quotation basis.
Tariffs
Rates are exclusive of tax (excluding VAT) and are charged per day of use, subject to annual review.
Prices include access to the platform’s instruments, the equipment and specific OctetRED96E plastic consumables.
Some specific consumables sush as SA or AR2G biosensors can be provided by the platform with extra charges. Other specific consumables must be provided by the user.
Any additional consumables supplied by the platform will incur extra charges.
Upon request, a quotation can be provided.
Location
The BLI platform is located on the EPN campus in Grenoble, within the CIBB building, room 001.
External users must obtain authorization to access the EPN campus; please contact platform staff before your visit.
Accommodation at the EPN campus Guest House can be arranged at the user’s expense; contact platform staff for assistance.
Use of the Platform
New Users
New users or users with new project must contact platform staff before any experimentation or instrument reservation. A preliminary meeting is mandatory to:
- Define the terms of platform use: collaboration or autonomous access.
- Discuss experimental conditions and establish the optimal strategy for the study.
- Schedule training and initial instrument use.
Users must agree to comply with the General Conditions and Operating Instructions (pdf).
Registered Users
Trained and registered users can book directly after checking the booking schedule.
Availability and Reservation
BLI calendar
Biophysic Platform
Institute of Structural Biology – UMR 5075 (CNRS-CEA-UGA)
71 Avenue des Martyrs
CS 10090
38044 Grenoble Cedex 9
France
Email: biophysics.facility@ibs.fr
Scientific Manager
Philippe Mas
Email: philippe.mas@ibs.fr
Phone: +33 (0)4 57 42 86 74
GETTING STARTED GUIDE
A usage guide for the OctetRED96e is available for download here (pdf).
WHAT TO BRING FOR EXPERIMENTS
You need
-
- Running buffer(s).
-
- Ligand samples at the highest concentrations (to be diluted in immobilization buffers).
-
- Analyte samples at the highest concentrations (to be diluted in running buffers).
-
- Regeneration buffers, if known.
-
- Biosensors.
-
- Calibrated pipettes and tips.
-
- Necessary plastic tubes (e.g., Falcon, Eppendorf).
-
- Relevant previous data and/or protocols.
Ordering consumables and kits
-
- Users must provide consumables specific to their experiments.
Consumables must be ordered from Sartorius (https://www.sartorius.com/en/products/biolayer-interferometry).
No other biosensors will be accepted.
- Users must provide consumables specific to their experiments.
WHAT THE PLATFORM PROVIDES
The BLI platform offers essential and common supplies for experiments on the OctetRED96e, including:
-
- The OctetRED96e instrument.
-
- 4°C refrigerator and -20°C freezer for sample storage.
-
- Vortex mixer and benchtop centrifuge.
-
- P20/Tween-20 and BSA (added to experimental buffers).
-
- Immobilization kits for amine coupling methods.
-
- Regeneration scouting kit.
-
- 96-wells plates for OctetRED96e.
-
- For external users: If you cannot bring laboratory supplies, you may borrow pipettes, tips, and other plastic or glass items from our laboratory.
-
- Filtered water solutions and instrument maintenance support.
HOW MUCH TIME DO I NEED?
The time needed to fully characterize your interaction depends on your biological system, experimental strategy, optimization process, and potential challenges. The duration can range from a few days to several weeks.
Below are estimated time requirements for traditional steps:
-
- Starting sequence: 5 to 10 minutes.
-
- Immobilization scouting: 30 to 60 minutes per ligand sample.
-
- Immobilization by amine coupling: 45 to 60 minutes per sample.
-
- Secondary capture: 20 to 30 minutes per sample.
-
- Pilot analyses: Half a day to several days, depending on the number of sample injections, concentration gradients, and the testing of contact and dissociation times, flow rates, and regeneration conditions.
-
- MCK (Multi-Cycle Kinetics)/Steady-State Analysis: 30 minutes to 1 hour or more, depending on kinetics and concentrations.
-
- Stop sequence: 5 to 10 minutes.
COMPUTING
-
- The platform provides computers dedicated to controlling each instrument, implementing experiments, acquiring and recording data, and conducting data analysis.
-
- All computers are equipped with regularly updated software essential for data monitoring and analysis.
ACKNOWLEDGEMENTS, PUBLICATIONS, AND VALORIZATION
Collaborations
For collaborative projects, platform staff must be listed as co-authors in peer-reviewed journals and other communications where SPR or BLI data are presented, as per the terms agreed upon prior to the project.
Service Access
Users must acknowledge the platform and its staff in all publications or communications that include SPR or BLI data. Please include the following acknowledgment text:
« This work used the platforms of the Grenoble Instruct-ERIC center (ISBG; UAR 3518 CNRS-CEA-UGA-EMBL) within the Grenoble Partnership for Structural Biology (PSB), supported by FRISBI (ANR-10-INBS-0005-02) and GRAL, financed within the University Grenoble Alpes graduate school (Écoles Universitaires de Recherche) CBH-EUR-GS (ANR-17-EURE-0003). The authors acknowledge the SPR/BLI platform scientific responsible, Philippe Mas, for his help and assistance. »
QUALITY
As part of its commitment to continuous improvement, the BLI technical platform adheres to a quality management system that has been ISO 9001:2015 certified since June 2011.
