The SAFe360 microscope from Abbelight and Olympus (SR-M4D). This new microscope was installed on the M4D imaging platform in 2021 and aims to reinforce our super-resolution capabilities (PALM/ dSTORM/ (DNA)PAINT and single particle tracking). The microscope allows 2D/3D multicolour imaging of fixed and living cells with a resolution of few tens of nanometers, as well as single particle tracking experiments with a localization precision up to 5-10 nm for bright organic fluorophores. The system is equipped with a laser combiner (405 nm, 488 nm, 532 nm, 561 nm, 640 nm et 730 nm), a module for homogeneous EPI/HiLo/TIRF illumination and two sCMOS Hamamatsu Orca Fusion cameras offering a large field of view and high acquisition speeds (reaching more than 100 frames per second). The microscope is highly automated and controlled via Abbelight’s NEO software. For a more detailed information, you can contact us or visit the manufacturer’s website:
https://www.abbelight.com/technology/nanoscopy
Detailed system specifications are available in the SR-M4D User Guide or upon a request.
Applications
– Single molecule localization microscopy (SMLM) imaging: 2D and 3D (astigmatism and SAF)
- PALM
- dSTORM
- (DNA)PAINT
– Multicolour acquisitions: classical emission separation and spectral demixing
– Single particle tracking (spt) on living cells (incubation chamber)
– Multicolour TIRF
Figure 1. R800 zDADA Alexa Fluor 647
Credits to C. Morlot, Q.-M. Nguyen
Figure 2. HeLa cell DNA labelling with Hoechst-JF549 (Janelia Farm)
Dedicated staff
Oleksandr Glushonkov (+33(0)4 57 42 86 85)
Jean-Philippe Kleman (+33(0)4 57 42 85 31)
Booking
