The epifluorescence imaging station (V-M4D, Olympus) is composed of an inverted Olympus IX83 fully motorized microscope, a 16-channel LED source, and equipped with a thermostatic incubation chamber for live cell imaging. Acquisition of images is achieved with a sCMOS camera (Hamamatsu Orca Flash4).
Detailed system specifications are available in the V-M4D User Guide or upon a request.
Applications
– DIC and phase contrast
– multichannel (multicolour) imaging
– 2D/3D (deconvoluted) fluorescence imaging of large zones (ROI stitching)
– time-lapse
Figure 1. Stitching 100x. FluoCells® (F6924 Thermo) contains bovine pulmonary artery endothelial (BPAE) cells stained with MitoTracker® Red CMXRos (red, mitochondria), Alexa Fluor® 488 phalloidin (green, F-actin) and DAPI (blue, nuclei)
Dedicated staff
Jean-Philippe Kleman (+33(0)4 57 42 85 31)
Oleksandr Glushonkov (+33(0)4 57 42 86 85)
Rose-Laure Revel-Goyet (+33(0)4 57 42 86 24)
Booking
