The home-built super-resolution setup (PALM-M4D) is based on an inverted Olympus IX81 microscope, equipped with two cameras, one sCMOS (Hamamatsu Orca Fusion BT) and one EMCCD (Photometrics Evolve 512), and eight lasers (355 nm, 405 nm, 488 nm, 532 nm, 561 nm, 643 nm, 730 nm and 885 nm). Micro-Manager v2.0 software is used to control the microscope with cameras and to perform image acquisitions, whereas a Labview code allows a precise lasers control. This microscope is recommended for more experienced users and can be modified/adapted on demand for a particular need or a scientific project. More details on the IBS web site.
Detailed system specifications are available in the PALM User Guide or upon a request.
Applications
– Single molecule localization microscopy (SMLM) imaging: 2D and 3D (astigmatism)
- PALM
- dSTORM
- (DNA)PAINT
– Single particle tracking (spt) on living cells (incubation chamber)
– Single molecule imaging: photophysics of individual fluorescent labels attached to a coverglass
– Switching kinetics of fluorescent labels at the ensemble level
Figure 1. Nuclear pore complexes of U2OS cells (Nup96-eGFP @GFP-AF64). Left – diffraction limited snapshot;
right – reconstructed dSTORM image
Dedicated staff
Oleksandr Glushonkov (+33(0)4 57 42 86 85)
Jean-Philippe Kleman (+33(0)4 57 42 85 31)
Booking
