The spinning disk confocal system has been updated in May 2022 (S-M4D; Olympus GATACA and Andor). It is based on a motorized Olympus IX81 microscope, equipped with a Nipkow disk (Yogokawa CSU-X1), six solid-state lasers for real-time confocal imaging of living cells and a photoconversion/ photoactivation / photoablation module (used, for example, in FRAP experiments). Images are collected with an EMCCD camera (Andor iXon). The confocal imaging has few advantages over the traditional epifluorescence technique, including improved resolution and higher contrast due to drastic reduction of the background and optical sectioning.
Detailed system specifications are available in the S-M4D User Guide or upon a request.
Applications
– 2D and 3D multicolour imaging with optical sectioning and piezo z-stage
– long time-lapse (low phototoxicity, incubation chamber with CO2, hardware focus stabilization)
– FRAP (fluorescence recovery after photobleaching)
– FRET (Forster resonance energy transfer)
– photodamage/ photoablation with the 355 nm laser
Figure 1. Membrane photodamage repair/ healing visualized by ALIX-eGFP recruitment to the damage site.
Credits: N. Miguet
Figure 1. Deinococcus radiodurans: membrane (NileRed, red) and nucleoid staining (Syto9, green).
Credits to J.P. Kleman, F. Lacroix and J. Timmins
Dedicated staff
Jean-Philippe Kleman (+33(0)4 57 42 85 31)
Oleksandr Glushonkov (+33(0)4 57 42 86 85)
Rose-Laure Revel-Goyet (+33(0)4 57 42 86 24)
